Structures of bacterial biosynthetic arginine decarboxylases

Arginine and ornithine decarboxylases, the polyamine biosynthetic enzymes of mung bean seedlings.

General properties and relative activities of l-arginine decarboxylase (ADC) (EC 4.1.1.19) and l-ornithine decarboxylase (ODC) (EC 4.1.1.17), two important enzymes in putrescine and polyamine biosynthesis, were investigated in mung bean (Vigna radiata L.) tissues. Both activities increase linearly with increasing concentrations of crude enzyme, but the increase in ADC activity is considerably g...

Activities of arginine and ornithine decarboxylases in various plant species.

In extracts from the youngest leaves of Avena sativa, Hordeum vulgare, Zea Mays, Pisum sativum, Phaseolus vulgaris, Lactuca sativa, and four pyrrolizidine alkaloid-bearing species of Heliotropium, the activities of ornithine decarboxylase, close to V(max), ranged between traces and 1.5 nanomoles per hour per gram fresh weight when based on putrescine formed during incubation with labeled ornith...

Assaying ornithine and arginine decarboxylases in some plant species.

A release of (14)CO(2) not related to ornithine decarboxylase activity was found in crude leaf extracts from Lycopersicon esculentum, Avena sativa, and especially from the pyrrolizidine alkaloid-bearing Heliotropium angiospermum when incubated with [1-(14)C]- or [U-(14)C]ornithine. The total (14)CO(2) produced was about 5- to 100-fold higher than that due to ornithine decarboxylase activities c...

Bacterial metabolism of arginine.

Biosynthetic Arginine Decarboxylase from Escherichia coli

Biosynthetic arginine decarboxylase of Escherichia coli has been purified 1,500-fold. The purified enzyme showed two bands on disc gel electrophoresis, both carrying out the decarboxylation of L-arginine. Electrophoresis in the presence of sodium dodecyl sulfate and sedimentation equilibrium ultracentrifugation was consistent with the enzyme being a tetramer of approximately 296,000 molecular w...